Effectiveness Test of Ketapang Leaf Extract (Terminalia Catappa L.) as a Hepatoprotective Agent in Mice (Mus musculus) Induced by Acetaminophen
Keywords:
Terminalia catappa L, hepatoprotective, acetaminophen, flavonoids, oxidative stress, DILIAbstract
Drug-induced liver injury (DILI) is a leading cause of acute liver failure, with acetaminophen being the most frequent etiological agent. Overdose induces formation of the toxic metabolite N-acetyl-p-benzoquinone imine (NAPQI), resulting in glutathione depletion, oxidative stress, and hepatocellular necrosis. This study aimed to evaluate the hepatoprotective effect of ethanolic extract of Terminalia catappa L. leaves in male mice (Mus musculus) with acetaminophen-induced acute liver injury. A post-test only control group design was employed using 20 mice divided into five groups: negative control (Na-CMC), positive control (N-acetylcysteine), and three treatment groups receiving T. catappa extract at doses of 200, 400, and 600 mg/kg BW. Acetaminophen was administered intraperitoneally at 500 mg/kg BW. Serum SGPT and SGOT levels were quantified spectrophotometrically, and phytochemical screening confirmed the presence of alkaloids, flavonoids, saponins, tannins, and terpenoids. Extract administration significantly (p < 0.05) reduced SGPT and SGOT levels compared to the negative control, with a clear dose-dependent trend; the 600 mg/kg BW group achieved biochemical parameters approaching those of the N-acetylcysteine group. The hepatoprotective effect is likely mediated by flavonoid-driven antioxidant and anti-inflammatory mechanisms. These findings indicate that T. catappa extract is a promising, safe, and cost-effective plant-based hepatoprotective agent, meriting further investigation toward clinical application as an adjunct therapy for drug-induced hepatotoxicity
Downloads
References
Afifah, N., Utami, D. P., & Widyastuti, E. (2023). Hubungan pengetahuan, sikap, dan perilaku perawatan kuku terhadap kesehatan kuku pada remaja putri. Jurnal Keperawatan Indonesia, 26(2), 123–132.
Ajeng, P., Rachmawati, E., & Pratama, A. R. (2020). Analisis kandungan kimia biji pacar air (Impatiens balsamina L.) dan potensinya dalam kosmetik herbal. Jurnal Farmasi Indonesia, 15(1), 45–53.
Amanda, R., & Huda, M. (2021). Potensi toksisitas bahan kimia pada kosmetik dekoratif. Jurnal Kesehatan Masyarakat, 9(3), 201–210.
Annisa, A. (2023). Tren pertumbuhan industri kosmetik global dan implikasinya di Indonesia. Jurnal Ekonomi dan Bisnis Global, 12(1), 56–68.
Astika, I. M., Sari, N. P., & Widana, I. K. (2022). Metode uji determinasi tanaman obat berbasis karakter morfologi. Jurnal Biologi Tropis, 22(4), 789–798.
Buana, P. Y., Rahayu, S., & Putra, A. H. (2024). Potensi senyawa aktif pacar air (Impatiens balsamina L.) sebagai bahan dasar kosmetik herbal. Jurnal Sains dan Teknologi Farmasi, 8(2), 99–107.
Fakhreza, R., & Muhammad, I. (2023). Analisis perkembangan industri kosmetik nasional pasca pandemi. Jurnal Manajemen Industri, 7(1), 55–66.
Furrahmi, N. (2019). Uji hedonik pada produk kosmetik herbal. Jurnal Penelitian dan Pengembangan Farmasi Indonesia, 3(2), 112–120.
Gyna, R. (2022). Fungsi dan manfaat nail polish dalam perawatan kuku. Jurnal Estetika dan Kecantikan, 4(1), 33–41.
Khalisah, S. (2022). Pemanfaatan daun inai (Lawsonia inermis L.) sebagai pewarna alami pada kosmetik dekoratif. Jurnal Teknologi Pangan dan Kesehatan, 5(2), 67–75.
Lestari, P., & Widayati, T. (2022). Perilaku konsumen dalam memilih produk kosmetik dekoratif. Jurnal Pemasaran dan Perilaku Konsumen, 14(3), 210–222.
Mikramin. (2022). Pengaruh viskositas terhadap daya sebar sediaan semi padat. Jurnal Sains Farmasi dan Klinis, 9(2), 77–84.
Ningsih, A. (2020). Tren penggunaan kosmetik dekoratif pada remaja. Jurnal Kesehatan dan Estetika, 6(2), 145–152.
Rahmiati, N., & Putri, D. M. (2022). Pengaruh pH terhadap kesehatan kuku dan kulit sekitar. Jurnal Kosmetologi Indonesia, 5(1), 22–29.
Rivera-Yañez, C. R., González-Ortega, A., & Martínez-Morales, F. (2021). Role of flavonoids in nail health and cosmetic formulations. International Journal of Cosmetic Science, 43(5), 432–440. https://doi.org/10.1111/ics.12740
SNI. (2016). Kosmetika – Persyaratan keamanan. Badan Standardisasi Nasional.
The Business Research Company. (2024). Nail polish global market report 2024. The Business Research Company.
Wandira, M., Kusuma, D., & Ratnawati, T. (2023). Distribusi partikel pada sediaan kosmetik berbasis herbal. Jurnal Teknologi Kesehatan, 11(2), 155–164.
Wasilah, R. (2020). Persepsi kecantikan dan dampaknya terhadap kepercayaan diri. Jurnal Psikologi Sosial, 8(1), 88–97.
