The Effect of Differences Time On The Macroscopic Picture Of Giemsa Staining Using Aquades Diluent

Ahdiah Imroatul Muflihah; Rian Anggia Destiawan; Anas Fadli Wijaya; Lenny Yulia Widia Sari; Queen Nurul Sufi; Alisa alfiya makki; Laila Camelia

Authors

Ahdiah Imroatul Muflihah Universitas dr. Soebandi
Rian Anggia Destiawan Faculty of Health Sciences, Universitas dr. Soebandi, Patrang, Indonesia
Anas Fadli Wijaya Faculty of Health Sciences, Universitas dr. Soebandi, Patrang, Indonesia
Lenny Yulia Widia Sari Faculty of Health Sciences, Universitas dr. Soebandi, Patrang, Indonesia
Queen Nurul Sufi Faculty of Health Sciences, Universitas dr. Soebandi, Patrang, Indonesia
Alisa alfiya makki Faculty of Health Sciences, Universitas dr. Soebandi, Patrang, Indonesia
Laila Camelia Faculty of Health Sciences, Universitas dr. Soebandi, Patrang, Indonesia

Keywords:

Blood, SADT, Giemsa

Abstract

Peripheral Blood Smear Preparation (SADT) is an examination used to see the structure and number of red and white blood cells, besides giemsa staining can be used for malaria examination. The quality of giemsa staining is influenced by one of which is the soaking time of the giemsa solution. The purpose of this study was to determine the quality of slides or blood smears based on the incubation duration of giemsa staining. This research method qualitatively performed giemsa staining on blood smears with different soaking times (10 minutes, 20 minutes, 30 meinit and 40 minutes) then compared with controls. Results showed that at minutes 10 and 20 the giemsa staining showed a smooth surface and no granules, at 30 and 40 minutes the giemsa staining showed a rough surface and many granules. The conclusion of this study was that 10 and 20 min giemsa soaking showed optimal staining of blood smear preparations.

References

Nugraha G. Panduan Pemeriksaan Laboratorium Hematologi Dasar. Jakarta: Trans Info Media; 2015.

Gandasubrata. Penuntun Laboratorium Klinik. Jakarta: Dian Rakyat; 2010.

Warsita N, Fikri Z, Ariami P. Pengaruh Lama Penundaan Pengecatan Setelah Fiksasi Apusan Darah Tepi Terhadap Morfologi Eritrosit. Jurnal Analis Medika Biosains (JAMBS) 2019;6:125. https://doi.org/10.32807/jambs.v6i2.145.

Febriyani R, Budi S. Kualitas Makroskopis dan Mikroskopis Morfologi Lekosit pada Sadt Berdasarkan Variasi Suhu Pengeringan. Jurnal Ilmiah Kesehatan 2020;15:245–50.

Ghofur A, Suparyati T, Fatimah S. Pengaruh Variasi Waktu Fiksasi Sediaan Apus Darah Tepi (SADT) pada Pengecatan Giemsa terhadap Morfologi Sel Darah Merah. Jurnal Kebidanan Harapan Ibu Pekalongan 2022;9:27–33. https://doi.org/10.37402/jurbidhip.vol9.iss1.171.

Diarti MW, Tatontos EY, Turmuji A. Larutan pengencer alternatif nacl 0,9 % dalam pengecatan giemsa pada pemeriksaan morfologi spermatozoa the alternative dilute solution of nacl 0.9% at the giemsa staining on the investigation the morphology of spermatozoa. Jurnal Kesehatan Prima 2016;10:1709–16.

Arianda D. Buku Saku Analis Kesehatan Revisi 5. Bekasi: Analis Muslim Publishing; 2015.

Islawati, Asriyani Ridwan, Rahmat Aryandi. Ekstrak Betasianin dari Umbi Bit (Beta vulgaris) sebagai Pewarna Alami pada Sediaan Apusan Darah Tepi. Jurnal Kesehatan Panrita Husada 2021;6:152–60. https://doi.org/10.37362/jkph.v6i2.644.

Teramoto A, Yamada A, Tsukamoto T, Kiriyama Y, Sakurai E, Shiogama K, et al. Mutual stain conversion between Giemsa and Papanicolaou in cytological images using cycle generative adversarial network. Heliyon 2021;7:e06331. https://doi.org/10.1016/j.heliyon.2021.e06331.

Aini N, Khasanah H, Husen F, Yuniati NI. Pewarnaan Sediaan Apusan Darah Tepi (Sadt) Menggunakan Infusa Bunga Telang (Clitorea ternatea). Jurnal Bina Cipta Husada Vol . XIX , No . 1 Januari 2023 Jurnal Kesehatan Dan Science , e-ISSN : I858-4616: 67–76.

Tahir KA, Haeria H, Febriyanti AP, Chadijah St, Hamzah N. Uji Aktivitas Antiplasmodium Dari Isolat Kulit Batang Kayu Tammate (Lannea coromandelica Houtt. Merr.) Secara In-Vitro. Jurnal Fitofarmaka Indonesia 2020;7:16–21. https://doi.org/10.33096/jffi.v7i1.591.

Nurjanah. Pewarnaan Sitologi pada Epitel Mukosa Menggunakan Giemsa Modifikasi 2020:6–11.

M. Arif. Penuntun Praktikum Hematologi. Makassar: Fakultas Kedokteran UNHAS; 2015.

Sahabhudin. Pengaruh Lama Pengecatan Sediaan Apus Darah Tipis Dengan Menggunakan Cat Giemsa Terhadap Morfologi Eritrosit. Universitas Muhammadiyah Semarang, 2015.

Jasin Maskoeri. Ilmu Alamiah Dasar. Jakarta: PT Raja Grafindo Persada; 2008.