Test of the Effectiveness of Moringa Oleifera Leaf Extract Cream on the Healing Process of Cut Wounds on the Skin Surface of Male Wistar Rats (Rattus Norvegicus)
Keywords:
Moringa Oleifera, Skin Surface, Healing Process, HistopathologicalAbstract
Everyone there must have been injured in some way. Moringa Oleifera leaves have significant antioxidant activity and are just one of many plant-based treatments used to treat pain and speed wound healing. This study aims to ascertain if a cream made from the extract of moringa leaves (Moringa oleifera) accelerates the recovery of damages caused by cuts. This study strategy employs an actual or laboratory experimental design based on a pre-post test with a control group. Twenty adult male Wistar rats (Rattus norvegicus) were used. Mice were acclimatized, phytochemicals were screened, and treatment and monitoring were performed using dermapen wounds during the investigation. The Kolmogorov-Smirnov test, One-Way Analysis of Variance (ANOVA), and LSD post hoc Test were used to examine the research data in SPSS. Results from a 14-day study on the effects of a cream containing Moringa leaf extract on the wound healing of male Wistar rats (Rattus norvegicus) led researchers to this conclusion. In a recent phytochemical study, flavonoids, saponins, tannins, and alkaloids, known to have therapeutic benefits due to their high antioxidant content, were found in Moringa leaf extract. The secondary metabolite chemicals found in Moringa leaf extract significantly affected wound healing, with the best effects shown at an extract level of 10%.
References
Adha, A., Dewana, W., Suyono, T., Chiuman, L., & Ginting, S. F. (2022). Testing Antioxidant And Antibacterial Activity Of Andaliman Fruit ( Zanthoxylum Acanthopodium Dc .) Ethanol Extract With Abts Acid ) And Minimum Resistant Concentration. International Journal of Health and Pharmaceutical Testing, 2(1), 188–196.
Afrylyani, Z., Rachmawati, J., & Hardi, E. (2022). Pengaruh Campuran Ekstrak Daun Kenikir dan Daun Sirih terhadap Penyembuhan Luka Sayat pada Mencit. J-KIP (Jurnal Keguruan Dan Ilmu Pendidikan), 3(2), 1–23.
Baquero, F., & Levin, B. R. (2021). Proximate and ultimate causes of the bactericidal action of antibiotics. Nature Reviews Microbiology, 19(2), 123–132. https://doi.org/10.1038/s41579-020-00443-1
Calsum, U., Khumaidi, A., & Khaerati, K. (2018). Aktivitas Ekstrak Etanol Kulit Batang Kayu Jawa (Lannea coromandelica) terhadap Penyembuhan Luka Sayat pada Tikus Putih (Rattus Norvegicus L.). Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) (e-Journal), 4(2), 113–118. https://doi.org/10.22487/j24428744.2018.v4.i2.11078
Campbell, D. T., & Stanley, J. C. (2015). Experimental And Quasi-Experiment Designs for Research (Vol. 29). Wilmington, Massachusetts, U.S.A: Cengage Learning. https://doi.org/10.1037/022808
Chakraborty, S., Majumder, S., Ghosh, A., Saha, S., & Bhattacharya, M. (2021). Metabolomics of potential contenders conferring antioxidant property to varied polar and non-polar solvent extracts of Edgaria darjeelingensis C.B.Clarke. Bulletin of the National Research Centre, 45(1). https://doi.org/10.1186/s42269-021-00503-3
Che Soh, N. A., Rapi, H. S., Mohd Azam, N. S., Santhanam, R. K., Assaw, S., Haron, M. N., … Ismail, W. I. W. (2020). Acute Wound Healing Potential of Marine Worm, Diopatra claparedii Grube, 1878 Aqueous Extract on Sprague Dawley Rats. Evidence-Based Complementary and Alternative Medicine, 2020(Mic). https://doi.org/10.1155/2020/6688084
Cusack, B., & Buggy, D. J. (2020). Anaesthesia, analgesia, and the surgical stress response. BJA Education, 20(9), 321–328. https://doi.org/10.1016/j.bjae.2020.04.006
Decker, A. M., Kapila, Y. L., & Wang, H. L. (2021). The psychobiological links between chronic stress-related diseases, periodontal/peri-implant diseases, and wound healing. Periodontology 2000, 87(1), 94–106. https://doi.org/10.1111/prd.12381
Djuwarno, E. N., Hiola, F., & Isa, I. (2021). Formulasi Sediaan Emulgel Ekstrak Daun Kelor (Moringa oleifera Lam) Dan Uji Aktivitas Antioksidan Menggunakan Metode DPPh. Indonesian Journal of Pharmaceutical Education, 1(1), 10–19. https://doi.org/10.37311/ijpe.v1i1.9947
Eka, S. R., Sahrial, H. I., Fikri, F., & Thohawi, E. P. M. (2016). Ekstrak etanol kayu secang (Caesalpinia sappan L.) secara topikal efektif pada kepadatan kolagen masa penyembuhan luka insisi tikus putih. Jurnal Medik Veteriner, 2(2), 1–23. Retrieved from https://e-journal.unair.ac.id/JMV
Erwiyani, A. R., Haswan, D., Agasi, A., & Karminingtyas, S. R. (2020). The Effect of Gel and Cream Dosage Forms of Moringa Leaves (Moringa oleifera Lamk) On The Reduction of Burns in Rats. Indonesian Journal of Pharmacy and Natural Product, 03(02), 41–52.
Fam, V. W., Charoenwoodhipong, P., Sivamani, R. K., Holt, R. R., Keen, C. L., & Hackman, R. M. (2022). Plant-Based Foods for Skin Health: A Narrative Review. Journal of the Academy of Nutrition and Dietetics, 122(3), 614–629. https://doi.org/10.1016/j.jand.2021.10.024
Grey, J. E., & Harding, K. G. (2009). ABC of Wound Healing (April, Vol. 332). Hoboken, New Jersey, USA: Blackwell Publishing.
Khafifah, E. R., Suwendar, & Fitrianingsih, S. P. (2020). Studi Literatur Tanaman yang Berkhasiat sebagai Penyembuh Luka Sayat. Prodi Farmasi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Univeristas Islam Bandung, Bandung, 6(2), 382–387.
Kurniawan, Pertiwi, A. T., & Lestari, I. T. (2021). Analisis Kadar Flavonoid Total Ekstrak Sirih Hijau (Piper betle L.). Journal of Islamic Pharmacy, 5(1), 80–85.
Nafi, R. M., Sasputra, I. N., & Rante, S. D. T. (2020). Uji Perbandingan Efektivitas Pemberian Ekstrak Daun Kelor (Moringa oleifera) dan Salep Gentamisin Terhadap Penyembuhan Luka Insisi Kulit Mencit (Mus Musculus). Cendana Medical Journal, 19(1), 108–117.
Notoatmodjo, S. (2018). Metodologi Penelitian Kesehatan (3rd ed.). Jakarta: Rineka Cipta.
Pamungkas, W. T., & Wahyuningsih, S. S. (2022). Efek Pemberian Ekstrak Etanol Daun Alpukat ( Persea Americana Mill ) Terhadap Penyembuhan Luka Bakar Pada Kelinci ( Oryctolagus Cunicullus ) . The Effect Of Giving Alpukat Leaves ( Persea Americana Mill ) Ethanol Extract On Current Wound Healing In Rabbit. Indonesian Journal On Medical Science, 9(1), 33–39.
Prastika, D. D., Setiawan, B., Saputro, A. L., Yudaniayanti, I. S., Wibawati, P. A., & Fikri, F. (2020). Effect of Shrimp Chitosan Topically on Collagen Density as Excision Wound Healing Parameter in Albino Rats. Jurnal Medik Veteriner, 3(1), 101–107. https://doi.org/10.20473/jmv.vol3.iss1.2020.101-107
Putra, N. R., Rizkiyah, D. N., Idham, Z., Salleh, L. M., Machmudah, S., & Yunus, M. A. C. (2021). Formulation and evaluation of a new semi-empirical model for solubility of plant extracts in supercritical carbon dioxide assisted by ethanol as co-solvent. Chemical Engineering Communications, 208(9), 1326–1334. https://doi.org/10.1080/00986445.2020.1771324
Ririn Agustina, Ajeng Dian Pertiwi, N. A. (2019). Efektifitas Salep Daun Sirih Hijau ( Piper Betle Linn ) Terhadap Luka. Pharmaceutical & Traditional Medicine, 3(1), 9–9. Retrieved from www.lppm-mfh.com lppm-politeknikmfh@gmail.com
Röhl, J., Zaharia, A., Rudolph, M., & Murray, R. Z. (2015). The Role Of Inflammation In Cutaneous Repair. Wound Practice & Research, 23(1), 8–15. Retrieved from the Walden Library databases
Sorg, H., Tilkorn, D. J., Hager, S., Hauser, J., & Mirastschijski, U. (2017). Skin Wound Healing: An Update on the Current Knowledge and Concepts. European Surgical Research, 58(1–2), 81–94. https://doi.org/10.1159/000454919
Srivastav, A. D., Singh, V., Singh, D., Giri, B. S., & Singh, D. (2021). Analysis of natural wax from Nelumbo nucifera leaves by using polar and non-polar organic solvents. Process Biochemistry, 106(November 2020), 96–102. https://doi.org/10.1016/j.procbio.2021.04.007
Suwarno, B., & Nugroho, A. (2023). Kumpulan Variabel-Variabel Penelitian Manajemen Pemasaran (Definisi & Artikel Publikasi) (1st ed.). Bogor: Halaman Moeka Publishing.
Syaputri, I., Girsang, E., & Chiuman, L. (2022). Test Of Antioxidant And Antibacterial Activity Of Ethanol Extract Of Andaliman Fruit ( Zanthoxylum Acanthopodium Dc .) With Dpph ( 1 . 1-Diphenyl-2- Picrylhydrazil ) Trapping Method And Minimum Inhibitory Concentration. International Journal of Health and Pharmaceutical Test, 2(2), 215–224. https://doi.org/https://doi.org/10.51601/ijhp.v2i2.36
Valizadeh, A., Shirzad, M., Pourmand, M. R., Farahmandfar, M., Sereshti, H., & Amani, A. (2021). Levofloxacin nanoemulsion gel has a powerful healing effect on infected wound in streptozotocin-induced diabetic rats. Drug Delivery and Translational Research, 11(1), 292–304. https://doi.org/10.1007/s13346-020-00794-5
Weller, C., & Sussman, G. (2006). Wound dressings update. Journal of Pharmacy Practice and Research, 36(4), 318–324. https://doi.org/10.1002/j.2055-2335.2006.tb00640.x
Xu, H., Wang, Y., Takashi, E., Kamijo, A., Miura, D., Karasawa, K., … Zhang, L. (2022). Predicting the different progressions of early pressure injury by ultraviolet photography in rat models. International Wound Journal, 19(4), 834–844. https://doi.org/10.1111/iwj.13681
Zakiya, R., Mulqie, L., & Fitrianingsih, S. P. (2019). Uji Aktivitas Ekstrak Etanol Daun Kelor ( Moringa oleifera Lam ) Terhadap Penyembuhan Luka Bakar Derajat II pada Mencit Swiss. Prosiding Farmasi, 5(2), 504–511.
Zhang, X., Zhang, Z., Shu, Q., Xu, C., Zheng, Q., Guo, Z., … Lu, C. (2021). Copper Clusters: An Effective Antibacterial for Eradicating Multidrug-Resistant Bacterial Infection In Vitro and In Vivo. Advanced Functional Materials, 31(14), 1–15. https://doi.org/10.1002/adfm.202008720
