The Efficacy of Soursop Leaf Extract as a Hepatoprotector in White Rats Rattus norvegicus Induced by the Antituberculosis Drugs Isoniazid and Pyrazinamide
Keywords:
Soursop leaf, isoniazid, pyrazinamide, hepar, histopathological pictureAbstract
The use of antituberculosis drugs isoniazid and pyrazinamide triggers hepatotoxicity. Soursop leaves have a hepatoprotective effect because they contain antioxidant compounds that can help inhibit and prevent oxidative damage to the liver. This study aims to determine the effect of soursop leaf extract on the histopathological appearance of rat liver induced by isoniazid and pyrazinamide. This research is a laboratory experimental research using design posttest only control group design. This study used 24 rats Wistar divided into four groups and given treatment for 14 days. The negative control group was not given soursop leaf extract. The positive control group was given isoniazid and pyrazinamide, the treatment group I was given isoniazid and pyrazinamide and 1 hour later continued with soursop leaf extract 72 mg/day giving soursop leaf extract 144 mg/day. The dose of isoniazid used was 189 mg/day, while the dose of pyrazinamide was 252 mg/day. Termination of rats was carried out on the 15th day to collect rat liver organs. Furthermore, histopathological examination of the rat liver was carried out. The results showed that the rats in the negative control group had normal histopathological features. In the positive control group, there was a picture of hydropic degeneration. The histopathological picture of the rat liver in the treatment group 1 showed a picture of parenchymatous degeneration and in the treatment group 2, the liver cells were normal. Administration of soursop leaf extract affected the histopathological picture of rat liver induced by isoniazid and pyrazinamide.
References
Kementrian Kesehatan RI. (2018). Tuberkulosis ( TB ). Vol. 1, infoDATIN.
Drain, P. K., Bajema, K. L., Dowdy D, Dheda K, Naidoo K, and Schumacher, S. G. (2018). Incipient and Subclinical Tuberculosis: a Clinical Review of Early Stages and Progression of Infection. Clin Microbiol Rev. 2018;1–24.
Hardhana B, Sibuea F, Widiantini W, editors. (2021). Profil Kesehatan Indonesia 2020. Vol. 48. Jakarta: Kementerian Kesehatan RI.
Goh, Z. H., Tee, J. K., and Ho, H. K. (2020). An Evaluation of the In Vitro Roles and Mechanisms of Silibinin in Reducing Pyrazinamide-and Isoniazid-Induced Hepatocellular Damage. Int J Mol Sci [Internet]. 2020 May 2 [cited 2022 May 3];21(10). Available from: /pmc/articles/PMC7279482/
Banjuradja, I., and Singh G. (2020). Mekanisme Hepatotoksisitas dan Tatalaksana Tuberkulosis pada Gangguan Hati. Indones J Chest. 2020;7(2):56.
Juliarta, I. G, Mulyantari, N. K., and Yasa, I W. (2018). Gambaran hepatotoksisitas ( ALT/AST) penggunaan obat antituberkulosis lini pertama dalam pengobatan pasien tuberkulosis paru rawat inap di RSUP Sanglah Denpasar tahun 2014. E-Jurnal Med. 2018;7(10):1–10.
Djauhari T. (2019). Pre Eliminary Study : Pengaruh Pemberian First Line Drug Antituberculosis Terhadap Jumlah Hydropic Swelling Pada Sel Hepar Tikus Putih Jantan (Rattus novergicus Strain Wistar) yang Diinduksi Selama dua Minggu”. Saintika Med. 2019;15(1):60.
Mutakin, M., Fauziati, R., Fadhilah, F. N., Zuhrotun A, Amalia R, Hadisaputri, Y. E. (2022). Pharmacological Activities of Soursop (Annona muricata Lin.). Molecules [Internet]. 2022 Feb 1 [cited 2022 May 3];27(4). Available from: /pmc/articles/PMC8878098/
Parapaga, V. F. S., Durry, M. F., and Lintong, P. M. (2018). Efek Pemberian Ekstrak Daun Sirsak (Annona muricata L.) terhadap Gambaran Histopatologik Hati Tikus Wistar (Rattus norvegicus) yang Diinduksi Rifampisin. J e-Biomedik. 2018;6(2):195–209.
Sari, W. N., Saebani, and Dhanardhono, T. (2018). Pengaruh Pemberian Butylated Hydroxytoluene (2,6-Di-Tert-Butyl-4-Methylphenol) Per Oral Dosis Bertingkat Terhadap Gambaran Histopatologis Hepar Tikus Wistar. Diponegoro Med J (Jurnal Kedokteran Diponegoro). 2018;7(2):1154–1165.
Extraction, T., Maseration, I., Surbakti, C. I., and Utara, S. (2019). Uji Mutu Ekstrak Daun Sirsak (Annona muricata Linn) yang di Ekstraksi Secara Maserasi dengan Pelarut Etanol 70 % Quality Test Of Extract Leaf Soursop (Annona muricata Linn). The Extraction In Maseration With. J Farm. 2019;1(1):20.
Rahmawanti, A, Nur, D, and Mukhlis, A. (2021). Jurnal Biologi Tropis Histopathological of Brain, Eye, Liver, Spleen Organs of Grouper Suspected VNN in Penyambuan Village, North Lombok. J Biol Trop. 2021;141–152.
Sijid, S. A., Muthiadin, C., Zulkarnain, Z, and Hidayat, A. S. (2020). Pengaruh Pemberian Tuak Terhadap Gambaran Histopatologi Hati Mencit (Mus musculus). Icr Jantan. J Pendidik Mat Dan Ipa. 2020;11(2):193.
Muhartono, Oktarlina, R. Z., and Purohita, N. S. (2019). Pengaruh Pemberian Minuman Ringan Berkarbonasi terhadap Gambaran Histopatologi Hepar Tikus Putih (Rattus norvegicus) Jantan Galur Sprague dawley The Effects of Carbonated Soft Drink Administration on Liver Histopathological Changes in Male Rats ( Rattus. Majority Gambaran Histopatologi Hati Tikus Wistar (Rattus norvegicus). Medical Journal of Lampung University, 8(1), 71–77. Retrieved from: https://juke.kedokteran.unila.ac.id/index.php/majority/article/view/2297
Yana, E. F., and Budijastuti, W. (2022). Gambaran Histopatologi Toksisitas Hepar Tikus Jantan (Rattus norvegicus) Pasca Pemberian Sirup Umbi Yakon (Smallanthus sonchifolius). LenteraBio Berk Ilm Biol. 2022;11(1):202–207.
Merdana, I. M., Kardena, I. M, Budiasa, K., and Gunawan, I. M. D. (2019). Histopathological Structure of White Rats Liver After Giving Ant Nest Extract Due To Induced Paracetamol Toxic Dose. Bul Vet Udayana. 2019;(21):14.
Rarangsari, N. E. (2015). Pengaruh Ekstrak Daun Sirsak (Annona muricata L.) Terhadap SOD dan Histologi Hepar Tikus (Rattus norvegicus) yang Diinduksi Aloksan. Undergraduate thesis, Universitas Islam Negeri Maulana Malik Ibrahim
