An Investigation on The Impact of Orally Administered Celery and Orange Juices on The Production of Collagen in Rats Exposed to Ultraviolet-B Light

Authors

  • Adli Arhani Master Study Program in Biomedical Sciences, Faculty of Medicine, Dentistry and Health Sciences, Prima Indonesia University, Medan
  • Fioni Fioni Master Study Program in Biomedical Sciences, Faculty of Medicine, Dentistry and Health Sciences, Prima Indonesia University, Medan
  • Irza Haicha Pratama Master Study Program in Biomedical Sciences, Faculty of Medicine, Dentistry and Health Sciences, Prima Indonesia University, Medan

Keywords:

Skin collagen, Celery extract, orange extract, Ultraviolet-B Light

Abstract

At the correct dosage, ultraviolet (UV) radiation has many positive health effects and is essential for the body. The flip side is that exposure to excessive amounts of ultraviolet light (UV) can be harmful. Repetitive exposure to UVB light causes reactive oxygen species (ROS) to be produced, reducing the production of antioxidant enzymes, increasing oxidative protein modification, and causing glycation and lipid peroxidation products to accumulate. Because of this, it's essential to take precautions to guard the skin from free radical damage and slow the aging process by employing antioxidants in various forms. In this study, rats were exposed to UVB light and then given either celery juice (Apium graveolens) or orange juice (Citrus) orally to see if it would increase the quantity of collagen in the rats. True experimentation, or study in a controlled laboratory setting, is the method of choice. Each of the three groups consisted of eight white rats, for a total of twenty-four rats. Due to their high concentrations of antioxidant components, the phytochemicals found in celery and orange juices were determined to have therapeutic potential in the study's conclusions. With a collagen density value of 3+ and a density of collagen fibers in the skin ranging from 50 to 90% per field of view, the group given orange juice had a more substantial impact on the collagen growth of rat skin tissue after exposure to UVB light compared to the group given celery juice.

References

Azzahra, H., Pujiastuti, P., & Purwanto. (2014). Potensi Ekstrak Kulit Buah Manggis (Garcinia mangostana L.) Buatan Pabrik Terhadap Peningkatan Aktivitas Mikrobisidal Sel Neutrofil yang Dipapar Streptococcus mutans (The Potency of Manufactured Mangosteen Peel Extract (Garcin-ia mangostana L.) Towards In. E-Jurnal Pustaka Kesehatan , 2(1), 161–166.

Campbell, D. T., & Stanley, J. C. (2015). Experimental And Quasi-Experiment Designs for Research (Vol. 29, Issue 4). Wilmington, Massachusetts, U.S.A: Cengage Learning. https://doi.org/10.1037/022808

Chen, Y., Yu, Q., & Xu, C. B. (2017). A convenient method for quantifying collagen fibers in atherosclerotic lesions by ImageJ software. International Journal of Clinical and Experimental Medicine, 10(10), 14904–14910.

Chou, H. Y., Lee, C., Pan, J. L., Wen, Z. H., Huang, S. H., Lan, C. W. J., Liu, W. T., Hour, T. C., Hseu, Y. C., Hwang, B. H., Cheng, K. C., & Wang, H. M. D. (2016). Enriched astaxanthin extract from haematococcus pluvialis augments growth factor secretions to increase cell proliferation and induces MMP1 degradation to enhance collagen production in human dermal fibroblasts. International Journal of Molecular Sciences, 17(6). https://doi.org/10.3390/ijms17060955

Djajanti, A. D., & Asfi, D. (2018). Uji Aktivitas Sediaan Krim Ekstrak Etanol Herba Seledri (Apium graveolens L.) terhadap Luka Sayat pada Kelinci (Oryctolagus cuniculus L.). Media Kesehatan Politeknik Kesehatan Makassar, XIII(2), 40–45. https://doi.org/10.32382/medkes.v13i2.671 40

Ekawati, N., & Wulandari, F. (2021). Review : Pengaruh Suplementasi Astaxhantin dalam Mencegah Photoaging. Generics: Journal of Research in Pharmacy, 1(2), 60–69. https://doi.org/10.14710/genres.v1i2.11147

Fahrurroji, A., & Riza, H. (2020). Karakterisasi Ekstrak Etanol Buah Citrus amblycarpa (L), Citrus aurantifolia (S.), dan Citrus sinensis (O.). Jurnal Farmasi Dan Ilmu Kefarmasian Indonesia, 7(2), 100–113.

Fernandez, T. L., Van Lonkhuyzen, D. R., Dawson, R. A., Kimlin, M. G., & Upton, Z. (2014). Characterization of a human skin equivalent model to study the effects of ultraviolet B radiation on keratinocytes. Tissue Engineering - Part C: Methods, 20(7), 588–598. https://doi.org/10.1089/ten.tec.2013.0293

Ghozali, I. (2018). Aplikasi Analisis Multivariate dengan Program IBM SPSS 25. In Badan Penerbit Universitas Diponegoro.

Gromkowska-Kępka, K. J., Puścion-Jakubik, A., Markiewicz-Żukowska, R., & Socha, K. (2021). The impact of ultraviolet radiation on skin photoaging — review of in vitro studies. Journal of Cosmetic Dermatology, 20(11), 3427–3431. https://doi.org/10.1111/jocd.14033

Guan, L. L., Lim, H. W., & Mohammad, T. F. (2021). Sunscreens and Photoaging: A Review of Current Literature. American Journal of Clinical Dermatology, 22(6), 819–828. https://doi.org/10.1007/s40257-021-00632-5

Gustiana, S., Mustariani, B. A. A., & Suryani, N. (2022). Photochemical Screening and Antibacterial Activity Test from Combination of Celery (Apium graveolens L.) and Moringa (Moringa Oleifera L.) Leaf Ethanol Extract as Facial Mask Active Ingredients. SPIN: Jurnal Kimia & Pendidikan Kimia, 4(1), 95–107. https://doi.org/10.1145/504405.504408

Hutauruk, H. P., Yamlean, P. V. Y., & Wiyono, W. (2020). Formulasi dan Uji Aktivitas Sabun Cair Ekstrak Etanol Herba Seledri (Apium graveolens L) Terhadap Bakteri Staphylococcus Aureus. Pharmacon - Jurnal Ilmiah Farmasi - UNSRAT, 9(1), 73–81. https://doi.org/10.35799/pha.9.2020.29275

Jubaidah, S., Indriani, R., Sa’adah, H., & Wijaya, H. (2018). Formulasi dan Uji Pertumbuhan Rambut Kelinci dari Sediaan Hair Tonic Kombinasi Ekstrak Daun Seledri (Apium graveolens Linn) dan Daun Mangkokan (Polyscias Scutellaria (Burm.F.) Fosberg). Jurnal Ilmiah Manuntung, 4(1), 8–14. https://doi.org/10.24815/jn.v0i0.11335

Juzeniene, A., & Moan, J. (2012). Beneficial effects of UV radiation other than via vitamin D production. Dermato-Endocrinology, 4(2), 109–117. https://doi.org/10.4161/derm.20013

Kendall, L. V., Owiny, J. R., Dohm, E. D., Knapek, K. J., Lee, E. S., Kopanke, J. H., Fink, M., Hansen, S. A., & Ayers, J. D. (2018). Replacement, Refinement, and Reduction in Animal Studies With Biohazardous Agents. ILAR Journal, 59(2), 177–194. https://doi.org/10.1093/ilar/ily021

Madonna, R., Novo, G., & Balistreri, C. R. (2016). Cellular and molecular basis of the imbalance between vascular damage and repair in ageing and age-related diseases: As biomarkers and targets for new treatments. Mechanisms of Ageing and Development, 159(May), 22–30. https://doi.org/10.1016/j.mad.2016.03.005

Marlina, R., & Mujahid, I. (2020). Efektifitas Jus Jeruk Dan Jus Tomat Terhadap Hipertensi Di Desa Tambaksogra Banyumas. Human Care Journal, 5(3), 786–797. https://doi.org/10.32883/hcj.v5i3.760

Matsumura, Y., & Ananthaswamy, H. N. (2004). Toxic effects of ultraviolet radiation on the skin. Toxicology and Applied Pharmacology, 195(3), 298–308. https://doi.org/10.1016/j.taap.2003.08.019

Mentari, I. N., Ariza, D., & Halid, I. (2020). Pemanfaatan Ekstrak Daun Seledri (Apium graveolens) Sebagai Antikoagulan Pengganti EDTA (Ethylene Diamine Tetraacetic Acid) Pada Pemeriksaan Jumlah Trombosit. Jurnal Penelitian Dan Kajian Ilmiah Kesehatan, 6(2), 192–198.

Notoatmodjo, S. (2022). Metodologi Penelitian Kesehatan (3rd ed.). Jakarta: Rineka Cipta.

NRC, N.-. (2011). Guide for the Care and Use of Laboratory Animals. In The Physiologist (8th ed., Vol. 39, Issue 4). Washington, D.C.: National Academy Press.

Rahayu, M. T., Afifah, A., & Muflikhah, K. (2022). Efek Pemberian Ekstrak Etanol Seledri (Apium graveolens L.) terhadap Kadar Asam Urat pada Tikus Putih (Sprague Dawley) Model Chronic Kidney Disease. Mandala Of Health, 15(1), 30. https://doi.org/10.20884/1.mandala.2022.15.1.5784

Reilly, D. M., & Lozano, J. (2021). Skin collagen through the lifestages: importance for skin health and beauty. Plastic and Aesthetic Research, 8. https://doi.org/10.20517/2347-9264.2020.153

Ribeiro, F. M., Volpato, H., Lazarin-Bidóia, D., Desoti, V. C., de Souza, R. O., Fonseca, M. J. V., Ueda-Nakamura, T., Nakamura, C. V., & Silva, S. de O. (2018). The extended production of UV-induced reactive oxygen species in L929 fibroblasts is attenuated by posttreatment with Arrabidaea chica through scavenging mechanisms. Journal of Photochemistry and Photobiology B: Biology, 178(November 2017), 175–181. https://doi.org/10.1016/j.jphotobiol.2017.11.002

Rosaini, H., Makmur, I., Putri, R. D., & Sidoretno, W. M. (2019). Formulasi , Pengujian Aktivitas Antioksidan dan Antibakteri Sediaan Masker Gel Peel Off Ekstrak Etanol Herba Seledri (Apium graveolens L .). Jurnal Farmasi Higea, 11(2), 133–144.

Rosita, N., Purwanti, T., & Agustin, A. (2010). Stabilitas Fisik dan Efektivitas Sediaan Tabir Surya Kombinasi Oksibenson dan Oktil Metoksisinamat dalam Basis Gel Carbomer 940 dengan Penambahan Asam Glikolat. Majalah Ilmu Kefarmasian, 7(2), 16–26. https://doi.org/10.7454/psr.v7i2.3453

She, J. M., Yuan, H. H., Zhang, J., Si, Z. N., & Lan, M. B. (2019). Protective effect of the Rubus chingii Hu. fruit extract on ultraviolet B-induced photoaging via suppression of mitogen-activated protein kinases (MAPKs) in vitro. Archives of Biological Sciences, 71(3), 541–550. https://doi.org/10.2298/ABS190321038S

Siahaan, E. R., Pangkahila, W., & Wiraguna, A. A. G. P. (2017). Krim ekstrak kulit delima merah (Punica granatum) menghambat peningkatan jumlah melanin sama efektifnya dengan krim hidrokuinon pada kulit marmut (Cavia porcellus) betina yang dipapar sinar UVB. Jurnal Biomedik (Jbm), 9(1), 7–13. https://doi.org/10.35790/jbm.9.1.2017.15313

Subchan, P., Sofyanti Putri, R., Itsnal Muna, N., Magdalena Hutapea, C., Cahyani, E., & Hidayah, N. (2022). Ekstrak Bunga Telang (Clitoria ternatea L.) Menghambat Peningkatan Ekspresi Gen MMP-1 pada Kulit Tikus Wistar yang Terpapar Sinar Ultraviolet B. Journal of Midwifery and Health Science of Sultan Agung, 2, 13–21. https://doi.org/10.30659/jmhsa.v1i2.22

Sugiyono. (2018). Metode penelitian kuantitatif, kualitatif dan kombinasi (mixed methods). In Alfabeta (2nd ed.). Alfabeta.

Suwarno, B., & Nugroho, A. (2023). Kumpulan Variabel-Variabel Penelitian Manajemen Pemasaran (Definisi & Artikel Publikasi) (1st ed.). Bogor: Halaman Moeka Publishing.

Veronica, E., Chrismayanti, N. K. S., & Dampati, P. S. (2021). Potensi Ekstrak Kastuba (Euphorbia pulcherrima) Sebagai Tabir Surya Terhadap Paparan Sinar UV Potential Extract of Poinsettia (Euphorbia pulcherrima) as a sunscreen against UV exposure. Journal of Medicine and Health, 3(1), 83–92.

Weichbrod, R. H., Thompson, G. A. (Heidbrink), & Norton, J. N. (2018). Management of Animal Care and Use Programs in Research, Education, and Testing (2nd ed.). Boca Raton: CRC Press Taylor & Francis Group.

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Published

2023-12-14

How to Cite

Arhani, A., Fioni, F., & Pratama , I. H. (2023). An Investigation on The Impact of Orally Administered Celery and Orange Juices on The Production of Collagen in Rats Exposed to Ultraviolet-B Light. Jurnal EduHealth, 14(04), 588–597. Retrieved from https://ejournal.seaninstitute.or.id/index.php/healt/article/view/3544