Antioxidant Activity Of Ethanol Extract Of Papaya Leaves (Carica Papaya L.) On Reducing Blood Glucose Levels Of Streptozotocin-Induced Male Rats

Utami Islamiati; Matra Novalia Palipadang; Almawati Podomi; Viola Putrika Alvinita Landu

Authors

Utami Islamiati Pelita Mas College of Pharmacy, W. Monginsidi Street No. 106A, Palu, Central Sulawesi, Indonesia
Matra Novalia Palipadang Pelita Mas College of Pharmacy, W. Monginsidi Street No. 106A, Palu, Central Sulawesi, Indonesia
Almawati Podomi Pelita Mas College of Pharmacy, W. Monginsidi Street No. 106A, Palu, Central Sulawesi, Indonesia
Viola Putrika Alvinita Landu Pelita Mas College of Pharmacy, W. Monginsidi Street No. 106A, Palu, Central Sulawesi, Indonesia

Keywords:

Antioxidant, Carica papaya L, Streptozotocin

Abstract

The purpose of this study was to determine the content and total amount of secondary metabolites and determine the antioxidant activity and the effect of ethanol extract of papaya leaves on glucose reduction induced by streptozotocin in male albino rats. Measurement of the total content of secondary metabolites and antioxidants was carried out using a UV-visible spectrophotometer using DPPH reagent, and 30 male albino rats were divided into 6 groups, with 3 groups as normal controls. The negative control group received the appropriate dose: 100 mg/kgBB, 200 mg/kgBB, and 300 mg/kgBB. Positive qualitative test results include secondary metabolites such as alkaloids, flavonoids, saponins, and tannins. Quantitative test results show a total alkaloid content of 0.4739% (equivalent to quinine), saponins 7.518% (equivalent to sapogenin), tannins 0.147% (equivalent to tannic acid), and flavonoids 1.653% (equivalent to quercetin). The IC50 result of the antioxidant activity of papaya leaf extract of 83.07 ppm is included in the strong category. Blood glucose measurement data showed that ethanol extract from papaya leaves effectively reduced blood sugar levels with an average value of 106 mg/dl at a dose of 100 mg/kg body weight.

References

A’yun, Q dan Laily, A. N. 2015. Analisis Fitokimia Daun Pepaya (Carica Papaya L) Di Balai Penelitian Tanaman Aneka Kacang dan Umbi, Kendalpayak, Malang. Prosiding. Seminar Nasional Konservasi dan Pemanfataan Sumber Daya Alam, 134 –137.

Dewi, N.P., 2020. Uji Kualitatif Dan Kuantitatif Metabolit Sekunder Ekstrak Etanol Daun Awar-Awar (Ficus septica Burm. F) Dengan Metode Spektrofotometri Uv-Vis. Jurnal Acta Holish Pharm, Vol 2(1), 16-24.

Ishak, A. 2018. Analisis Fitokimia Dan Uji Aktivitas Antioksidan Biskuat Biji Labu Kuning (Curcubita sp). Sebagai Snack Sehat. Skripsi program studi ilmu gizi, Fukultas Kesehatan Masyarakat, Universitas Hasanudin Makassar,1-101.

Mahatriny, N. N., Payani, N. P. S., Oka, I. B. M., dan Astuti, K. W. 2014. Skrinning Fitokimia Ekstrak Etanol Daun Pepaya (Carica papaya L) Yang Diperoleh Dari Daerah Ubud, Kabupaten Gianyar, Bali. Jurnal Farmasi Udayana, Volume 3(1): 8 –13.

Nanggu, Y. Pura, H. 2016. Uji Aktivitas Antioksidan Menggunakan Metode Radikal DPPH (1,1-DIFENIL-2-PIKRILHIDRAZIL) Dan Penetapan Kadar Fenolik Total Fraksi Etil Asetat Ekstrak Etanol Daun BenaluScurrula ferruginea (Jack) Danser pada Tanaman Tabebuia aurea (Manso) Benth. & Hook.

Pratama, M., Raiz, R., & Vivien, S., R. 2019. Analisis Kadar Tanin Total Ekstrak Etanol Bunga Cengkeh (Syzygium Aromaticum L.) Menggunakan Metode Spektrofotometri Uv-Vis, Jurnal Fitofarmaka Indonesia, 6(2), 368–373.

Reni, E., Y. 2018. Pengantar Radikal Bebas Dan Antioksidan, CV Budi Utama: Yogyakarta.

Riza, M., M. 2016. Dasar –Dasar Fitokimia, CV.Trans Info Media: Jakarta Timur.

Sankarganesh, P., Baby Joseph., Ganesh Kumar. A., Lanjiam. S., dan Srinivasan T. 2018. Phytomedicinal Chemistry and Pharmacognostic Value of Carica papaya L., Leaf. Journal of pure and Applied Microbiology, 2(2).

Surya, A. 2017. Uji Aktivitas Antioksidan Pada Ubi Jalar Kuning (Ipomea batatas L) Dengan Metode DPPH (1,1 Difenil –2-Pikrilhidrazil). Jurnal ICA (Indonesia Chemia Acta ), 4(1): 12 –16.

Suyudi, M. 2017. Pengaruh Ekstrak Etanol Daun Sukun (Artocarpus altilis) ( Parkinson ex F.A.Zorn) Fosberg) Terhadap Kadar Ureum Dan Kreatinin Pada Tikus Putih Jantan (Rattus novergicus) hiperkolestrollemia-Diabtes. Program Studi S1 Farmasi Sekolah Tinggi Ilmu Farmasi Stifa Pelita Mas : Palu.

Tandi, J., Melinda, B., Purwantari, A., Widodo, A. 2020. Analisis Kualitatif dan Kuantitatif Metabolit Sekunder Ekstrak Etanol Buah Okra (Abelmoschus esculentus L. Moench) dengan Metode Spektrofotometri UV-Vis. KOVALEN:Jurnal Riset Kimia. 6(1): 74-80.

Tandi, J., Mulyani, S., Tibe, F., dan Wayan. 2016. Uji Efek Ekstrak Etanol Bawang Dayak (Elrutherine Bulbosa (Mill) URB) Sebagai Antihiperkolestrolemia, Prosiding Seminar Nasional Tumbuhan Obat Indonesia, 20–21.

Tandi, J., Dwianita, C., dan Dermiati, T. 2017. Pengaruh pemberian ekstrak daun talas (Colocasia esculenta (L.) Schott) terhadap penurunan kadar kolesterol total darah tikus putih jantan (Rattus norvegicus) yang diinduksi STZ. Farmakologika Jurnal Farmasi,14(2), 84–90.

Tuntun, M. 2016. Uji Efektivitas Ekstrak Daun Papaya (Carica papaya L)Terhadap Pertumbuhan Bakteri Escherichia coli dan Staphylococcus aureus, Jurnal Kesehatan, 7(3): 497 –502.