Antidiabetic Effects Of Robusta Coffee Bean Ethanol Extract (Coffea Canephora) In Diabetic Mice With Alloxan Induction

Ayu Wulandari; Sholihatil Hidayati; Dina Trianggaluh Fauziah

Authors

Ayu Wulandari Pharmacy Study Program, Faculty of Health Sciences, Universitas dr. Soebandi, Indonesia
Sholihatil Hidayati Pharmacy Study Program, Faculty of Health Sciences, Universitas dr. Soebandi, Indonesia
Dina Trianggaluh Fauziah Pharmacy Study Program, Faculty of Health Sciences, Universitas dr. Soebandi, Indonesia

Keywords:

Coffea canephora, blood glucose, alloxan

Abstract

Non-communicable diseases are a group of diseases which in themselves become a public health burden because they are widespread, one of which is diabetes mellitus (DM). Robusta coffee bean (Coffea canephora) is one of the medicinal plants that can be efficacious in lowering blood glucose levels. The purpose of this study was to determine the effect of Robusta Coffee Beans (Coffea canephora) on blood glucose levels in alloxan-induced mice. This study used a type of laboratory experimental study using male mice (Muss musculus L.) Bulb/C strain. There were 6 groups randomized and treated Robusta Coffee Bean Ethanol Extract (RBEE) with dose 100 mg/kg.bw, dose 200 mg/kg.bw, dose 400 mg/kg.bw, CMC Na 0.5% and metformin 195 mg/kg.bw. The treatment is given orally for 14 days. Blood glucose level data was analyzed using the ANOVA one-way parametric test with a confidence level of 95%. After being given treatment, the blood glucose level in negative control group (545.7 mg/dl), positive control (130.25 mg/dl), RBEE 100 mg/kg.bw (296.5 mg/dl), RBEE 200 mg/kg.bw (211.5 mg/dl), RBEE 400 mg/kg.bw (147.25 mg/dl), so that the percent decrease in blood glucose levels in RBEE 100 mg/kg.bw (27.10%), RBEE 200 mg/kg.bw (50.14%), RBEE 400 mg/kg.bw (68.00%), CMC Na (-53.60%), metformin (71.11%). The results showed that there was an effect on reducing blood glucose levels in mice significantly compared to the negative control group. Robusta coffee beans (Coffea canephora) have the activity of lowering blood glucose levels so that it can be developed as an antidiabetic.

References

Chairunnisa, S., Wartini, N.M., & Suhendra, L. (2019). Influence temperature and time maceration to characteristics extract leaf bidara (Ziziphus mauritiana L.) as source of saponins. Journal Agro-Industry Engineering and Management ISSN , 2503, 488X.

Dewi, NP, Mangela, PG, & Masyita, AA (2019). Testing the Effects of Majapahit Leaf Fractions to Decline Rat Blood Glucose Streptozotocin-Induced Whiteness. Pharmacologika : Journal Pharmacy, 16 (01), 91–102.

Djunarko, I., Anggal, FD, Sugianto, EAW, Rahayuningsih, KAM, Ivanka, FG, Wea, KCS, & Utomo, LS (2022). Leaf Soursop Annona Muricata L. as Antihyperglycemic. Journal Pharmaceuticals , 11 (1), 7–22.

Fahmi, M. (2022). Influence Giving Coffee to Reducing Blood Sugar Levels in DM Sufferers in Sungai Pinang Village, Working Area Mining. Health Center 2021/2022. 1 (1), 1–23.

Furqan, M., & Nurman , S. (2020a). Polar Extract of Arabica Green Coffee (Coffea arabica L.) as Antihyperglycemia in Mice (Mus musculus). Journal of Healthcare Technology and Medicine, 6 (2), 1323–1331.

Furqan, M., & Nurman, S. (2020b). Green coffee polar extract arabica (Coffea arabica L.) as antihyperglycemia in mice (Mus musculus). Journal Of Healthcare Technology and Medicine, 6 (2), 1323–1331.

Hamdani, I., & Nurman, S. (2020). Extract Ethanol of Arabica Green Coffee (Coffea arabica L.) as Antihyperglycemia in Mice (Mus musculus). Journal Indonesian Pharmacy, 140–147.

Handajani, F. (2021). Method selection and creation several animal models disease in research experimental. Zifatama Champion.

Hidayati, S. (2021). Antidiabetic Activity of Peperomia pellucida In Streptozotocin-Induced Diabetic Mice. Journal Pharmacy Galenika (Galenika Journal of Pharmacy) (e-Journal), 7 (2), 120–130. https://doi.org/10.22487/j24428744.2021.v7.i2.15429

Kartikasari, D., Rahman, IR, & Ridha, A. (2022). Phytochemical test on leaves kesum (Polygonum minus Huds.) from West Kalimantan. Journal Human Indonesian Pharmacy, 5 (1), 35–42.

Kurniawaty, E., & Lestari, E.E. (2016). Effectiveness Test Leaf Star fruit Wuluh (Averrhoa bilimbi L.) as Diabetes Mellitus Treatment The Effectiveness Test for Wuluh Extract Starfruite Leaf (Averrhoa bilimbi L .) as Diabetes Mellitus Treatment. Majority, 5 (2), 32.

Latief, M., Tarigan, IL, Sari, PM, Aurora, FE (2021). Activity Antihyperuricemia Extract Ethanol Leaf Sungkai (Peronema canescens Jack) in mice White Male. Pharmacon : Journal Indonesian Pharmacy, 18 (1), 23–37.

Rahmadyta Syafitri. (2020). Potency Extract Guava Leaves (Psidium guajava) Against Streptococcus mutans As Antibacterial. 21 (1), 1–9.

Rangkuti, YAP (2022). Effectiveness Test Infusion Sidikalang Coffee Beans (Coffea canephora var. robusta ) In Reducing Blood Sugar Levels in Wistar Rats (Rattus norvegicus L).

Riskesdas. (2018). Key Results of Basic Health Research. Ministry of Health of the Republic of Indonesia, 1–100.

Sukara, MA, Farid, N., Yusuf, M., & Yustikawati, Y. (2023). Effectiveness Infusion Skin Javanese Wood Trunk (Lannea Coromandelica) Against Decreased Blood Glucose Levels. Journal Promotional Preventive, 6 (1), 145–157.

Sulistyawati, R., Nurani, LH, Hidayati, S., Mursyidi, A., & Mustofa. (2017). Standardization Quality Fraction Ethyl Acetate Leaf Moringa (Moringa oleifera Lamk.). The 6th University Research Colloquium 2017 Muhammadiyah University of Magelang, 67–72.

Wadja, H., Rahman, H., & Supriyatni, N. (2019). Related Factors with Incidence of Diabetes Mellitus at UPTD Diabetes Center Ternate City in 2018. Journal Biosciences, 1 (01), 38–45.