Hepatoprotective Effect of Ethanol Extracted Clove Flower (Syzygium Aromaticum) on Wistar Rat’s Hepatocytes Induced by  Carbon Tetrachloride (CCl4)

Linlin Haeni; Dewi Ratih Handayani; Kautsar Balda Fauzan

Authors

Linlin Haeni Laboratorium Histologi, Fakultas Kedokteran Universitas Jenderal Achmad Yani, Cimahi, Jawa Barat
Dewi Ratih Handayani Departemen Biokimia, Fakultas Kedokteran Universitas Jenderal Achmad Yani, Cimahi, Jawa Barat
Kautsar Balda Fauzan Laboratorium Histologi, Fakultas Kedokteran Universitas Jenderal Achmad Yani, Cimahi, Jawa Barat

Keywords:

Carbon tetrachloride, clove flowers, hepatocyte

Abstract

Based on WHO, the total death caused only by alcohol causing liver cirrhosis complications was estimated at 637,000 people in 2016. Based on Riskesdas 2018, 3% of Indonesians consumed alcohol 0.3% excessively. One of the liver damage prevention due to alcohol was by consuming plant antioxidants. Ethanol extracted clove flowers (EEBC) contains antioxidant substances such as eugenol and flavonoids which protect hepatocytes from free radicals. The aim of this research was to determine the hepatoprotective effect of ethanol extracted clove flower on hepatocytes induced by carbon tetrachloride (CCl4). Trichloromethyl from CCl4 metabolism by P-450 enzymes caused lipid peroxidation of hepatocyte cell membranes This research was divided into 5 groups with 5 Wistar rats in each group. The group categories were negative control (K1), positive control (K2), 750 mg/KgBW dose EEBC (K3), 875 mg/KgBW dose EEBC (K4), 1000 mg/KgBW dose EEBC (K5). The research procedure was divided into 2 phases. First, administration of extract for 7 days, second, induction of CCl4 on the 8th day. On 9th day, rats were terminated and their livers were taken for histologic preparations. The results showed that K5 had the lesser destruction than positive control but still under negative control, and K3 and K4 almost the same as positive control. In conclusion, ethanol extracted clove flowers had hepatoprotective effect towards hepatocytes of induced CCl4 Wistar rats. Low and moderate doses of EEBC did not have a statistical effect, apparently because the induction of CCl4 was too strong compared to previous studies that used paracetamol.

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