Antibacterial Activity Test of Nipa Palm Leaf Extract (Nypa fruticans) Against Staphylococcus epidermidis
Keywords:
Nypa fruticans, antibacterial, , Staphylococcus epidermidisAbstract
Skin disorders are a common health problem and can be caused by pathogenic bacterial infections. Staphylococcus epidermidis bacteria are known to play a role in various infections that can affect the skin surface. The use of natural ingredients as alternative antibacterial agents is crucial, one of which is nipah leaves (Nypa fruticans), which contain various secondary metabolites with potential antibacterial activity. This study aimed to determine the antibacterial activity of nipah leaf ethanol extract against two test bacteria and to determine the most effective concentration. Antibacterial testing used the disc diffusion method at concentrations of 200 μg/disc, 300 μg/disc, and 400 μg/disc, with chloramphenicol as a positive control and DMSO as a negative control. The results showed that nipah leaf extract formed a zone of inhibition at all tested concentrations. The largest zone of inhibition was found at a concentration of 400 μg/disc, measuring 8,2 mm against Staphylococcus epidermidis, which is classified as having medium inhibitory activity.
References
Adriana, L., Dewi, C., & Nasir, N. H. (2023). Uji Aktivitas Antibakteri Ekstrak Etanol Daun Keji Beling
(Strobilanthes crispa BI) Terhadap Pertumbuhan Bakteri Staphylococcus epidermidis dan Pseudomonas aeruginosa. Jurnal Pharmacia Mandala Waluya, 2(3),162–174.
Diggle, S. P., & Whiteley, M. (2020). Microbe profile: Pseudomonas aeruginosa: Opportunistic pathogen and lab rat. Microbiology (United Kingdom), 166(1), 30–33.
Mahdani, W., X, S. R., & X, M. A. (2023). Evaluasi Kejadian Infeksi pada Pasien Luka Bakar yang Dirawat Inap di RSUD dr. Zainoel Abidin. Journal of Medical. Science, 3(2), 71–79.
Alfadli, R., & Khairunisa, S. (2024). Prevalensi Penyakit Kulit Infeksi dan Non-infeksi di Poliklinik Kulit dan Kelamin RSUD Jagakarsa Periode Februari 2023 – Januari 2024 Prevalence of Infectious and Non-infectious Skin Diseases in the Dermatology and Venereology Outpatient Department at Jag. 30(3), 151–156.
Khafid, A., Wiraputra, M. D., Putra, A. C., Khoirunnisa, N., Putri, A. A. K., Suedy, S. W. A., & Nurchayati, Y. (2023). UJi Kualitatif Metabolit Sekunder pada Beberapa Tanaman yang Berkhasiat sebagai Obat Tradisional. Buletin Anatomi Dan Fisiologi, 8(1), 61–70.
Subiandono, E., Heriyanto, N. M., & Karlina, E. (2016). Potensi Nipah (Nypa fruticans (Thunb.) Wurmb.) sebagai Sumber Pangan dari Hutan Mangrove. Buletin Plasma Nutfah, 17(1), 54.
Gamah, G., Nastiti, K., & Aryzki, S. (2023). Profil Senyawa Alkaloid Dengan Metode Spektroskopi
Inframerah (FTIR) Dan Penetapan Kadar Total Alkaloid Dari Ekstrak Daun Jarak Pagar (Jatropha curcas .L). Journal Pharmaceutical Care and Sciences, 4(1), 168–181.
Yuan, G., Guan, Y., Yi, H., Lai, S., Sun, Y., & Cao, S. (2021). Antibacterial activity and mechanism of plant flavonoids to gram-positive bacteria predicted from their lipophilicities. Scientific Reports, 11(1), 1–15.
Effendi, I., Rifai, M., Fazira Siregar, R., Syawal, H., Suriani, D. T., Yos-waty, D., & Riza, S. (2022).
Antibacterial activity of Nypa fruticans leaf extract to Aeromonas hydrophila, Pseudomonas acruginosa and Escherichia coli. Idesia (Arica), 40(3), 119–125.
Imra, Tarman, K., & Desniar. (2016). Aktivitas Antioksidan Dan Antibakteri Ekstrak Nipah (Nypa
fruticans) Terhadap Vibrio sp. Isolat Kepiting Bakau (Scylla sp.) Antioxidant and Atibacterial Activities of Nipah (Nypa fruticans) against Vibrio sp . Isolated From Mud Crab (Scylla sp.). 19(3), 241–250.
Putri, R. N., Wahidah, S. N., Hafidz, I. T. Al, & Faisal. (2023). Uji Daya Hambat Antimikroba Secara Difusi Sumuran dan Difusi Paper Disk Potential. Era Sains : Journal of Science, Engineering and Information Systems Research, 1(4), 2023.
Depkes, RI, (2000). Parameter Standar Umum Ekstrak Tumbuhan Obat. Departemen Kesehatan Republik Indonesia.
Abriyani, E., Gunarti, N. S., Oktaviani, S. P., & Amal, S. (2023). Skrining Fitokimia Dan Uji Antioksidan Ekstrak Daun Kangkung Pagar (Ipomoea carnea Jacq). Jurnal Buana Farma, 3(1), 37–40.
Nurhayati, L. S., Yahdiyani, N., & Hidayatulloh, A. (2020). Perbandingan Pengujian Aktivitas Antibakteri Starter Yogurt dengan Metode Difusi Sumuran dan Metode Difusi Cakram. Jurnal Teknologi Hasil Peternakan, 1(2), 41.
Rizki, S. A., Latief, M., & Rahman, H. (2021). Uji aktivitas antibakteri ekstrak n-heksan, etil asetat, dan etanol daun durian (durio zibethinus linn.) terhadap bakteri propionibacterium acnes dan staphylococcus epidermidis. Jurnal Mahasiswa Farmasi, 442–457.
Shafira, A. D., Lubis, M. S., Dalimunthe, G. I., & Mambang, D. E. P. (2023). Perbandingan Daya Hambat Terhadap Pertumbuhan Bakteri Staphylococcus Epidermidis Antara Serbuk Simplisisa Kulit Daun & Daging Daun Lidah Buaya (Aloe vera(L.)Burm.f). Farmasainkes, 3(1), 71–77.
Balouiri, M., Sadiki, M., & Ibnsouda, S. K. (2016). Methods for in vitro evaluating antimicrobial activity: A review. Journal of Pharmaceutical Analysis, 6(2), 71–79.
Otto, M. (2009). Staphylococcus epidermidis—the “accidental” pathogen. Nature Reviews Microbiology, 7(8), 555–567.
Cowan, M. M. (1999). Plant products as antimicrobial agents. Clinical Microbiology Reviews, 12(4), 564–582
Cushnie, T. P. T., & Lamb, A. J. (2011). Recent advances in understanding the antibacterial properties of flavonoids. International Journal of Antimicrobial Agents, 38(2), 99–107.
Madigan, M. T., Bender, K. S., Buckley, D. H., Sattley, W. M., & Stahl, D. A. (2018). Brock Biology of Microorganisms (15th ed.). Pearson Education.
