Antioxidant Activity Test of Peel-off Mask of Ethanol Extract of Kitolod Leaves (Isotoma Longiflora L)
Keywords:
Kitolod leaves (isotoma longoflora L.), peel-off mask, antioxidant.Abstract
Free radicals are one of the main causes of skin cell damage, resulting in premature aging. Antioxidants play an important role in neutralizing free radicals, thereby maintaining skin health. Kitolod leaves (Isotoma longiflora L.) are known to contain flavonoids, alkaloids, saponins, and polyphenols that have antioxidant potential. This study aims to formulate 70% ethanol extract of kitolod leaves into a peel-off gel mask preparation and evaluate its physical stability and antioxidant activity. The research method is experimental with variations in extract concentration of 5%, 10%, and 15%. Physical evaluation of the formulation included organoleptic testing, homogeneity, pH, viscosity, spreadability, adhesion, and drying time, while antioxidant activity was tested using the DPPH method. The results showed that all formulations met the characteristics of a good peel-off mask preparation and remained stable for 14 days of storage. The antioxidant activity test using the method showed that the IC50 value of kitolod leaf extract was 52.84 ppm, which is classified as strong. Thus, the peel-off gel mask containing kitolod leaf extract has the potential to be developed as a natural cosmetic product with good antioxidant activity.
References
Beringhs, A. O. R., Rosa, J. M., Stulzer, H. K., Budal, R. M., & Sonaglio, D. (2013). Green clay and aloe vera peel-off facial masks: Response surface methodology applied to the formulation design. AAPS PharmSciTech, 14(1), 445–455. https://pubmed.ncbi.nlm.nih.gov/23381175/
Garg, A., Aggarwal, D., Garg, S., & Singla, A. K. (2002). Spreading of semisolid formulations: An update. Pharmaceutical Technology North America, 26(9), 84–90.
Hidayati, N., Styawan, A. A., & Khotimah, A. K. (2020). Formulation and physical properties test of ethanol extract syrup of breadfruit leaves (Artocarpus altilis (Parkinson ex F.A. Zorn) Fosberg). Jurnal Ilmiah Farmasi, 9(2), 45–51.
Gultom, I. (2019). Formulation of peel-off gel mask preparation from California papaya (Carica papaya L.) fruit juice [Doctoral dissertation, Helvetia Health Institute]. Helvetia Repository.
Lambers, H., Chapin III, F. S., & Pons, T. L. (2008). Plant physiological ecology. Springer Science & Business Media.
https://doi.org/10.1007/978-0-387-78341-3
Lestari, S., Septiyani, B. N., Proklamasiningsih, E., & Hernayanti, H. (2024). Flavonoid content and antioxidant activity of kitolod (Hippobroma longiflora L.) at different growing altitudes. LenteraBio: Scientific Periodical of Biology, 13(2), 212–218.
https://journal.unesa.ac.id/index.php/lenterabio/article/view/23498
Muslihin, A. M., & Astuti, R. A. (2022). Analysis of antioxidant activity of neem leaf extract fractions (Azadirachta indica A. Juss) using the DPPH method. Jurnal Ilmu Farmasi Indonesia, 20(3), 3635–3644.
Muslihin, A. M., Rifai, Y., & Rante, H. (2022). Isolation and identification of endophytic fungi producing antioxidant compounds from Azadirachta indica A. Juss based on the 18s rRNA gene. Jurnal Farmasi dan Sains Terapan, 45(1), 3635–3644.
Nafiah, S. R., Fitraneti, E., Rizal, Y., Primawati, I., & Hamama, D. A. (2024). The effect of ultraviolet light exposure on skin health and prevention efforts: A literature review. Scientific Journal, 3(3), 185–194.
Pratiwi, L., & Wahdaningsih, S. (2018). Formulation and antioxidant activity of peel-off gel facial mask with papaya (Carica papaya L.) methanol extract. Pharmacy Medical Journal (PMJ), 1(2), 45–52.
DOI : 10.35799/PMJ.1.2.2018.21643
Pisoschi, A. M., & Pop, A. (2015). The role of antioxidants in the chemistry of oxidative stress: A review. European Journal of Medicinal Chemistry, 97, 55–74. https://doi.org/10.1016/j.ejmech.2015.04.040
Rahmayani, D., & Sari, D. (2023). Testing of flavonoid and antioxidant compound levels in ethanol extract of watercress (Nasturtium officinale R. Br) using UV-Vis spectrophotometry method. Nutrition Science and Health Research, 1(2), 21–27.
Rowe, D. (Ed.). (2009). Chemistry and technology of flavors and fragrances. John Wiley & Sons.
Shahidi, F., & Zhong, Y. (2015). Measurement of antioxidant activity. Journal of Functional Foods, 18, 757–781. https://doi.org/10.1016/j.jff.2015.01.047
Slavtcheff, C. S. (2000). Cosmetic composition for facial skin masks. Indonesia Patent No. 4913.
Soltif, N. (2025). Formulation and activity of peel-off gel mask with sea grape (Caulerpa racemosa) extract and honey (Mel depuratum) combination as antioxidant [Doctoral dissertation, Muhammadiyah University of Education, Sorong].
Sukmawati, N. M. A., Arisanti, C. I. S., & Wijayanti, N. P. A. D. (2013). The effect of variations in the concentration of PVA, HPMC, and glycerin on the physical properties of a peel-off gel facial mask made from 96% ethanol extract of mangosteen (Garcinia mangostana L.) peel. Udayana Pharmaceutical Journal, 2(3), 279–286.
Suyatmi, Saleh, C., & Pratiwi, D. R. (2019). Phytochemical testing and antioxidant activity test (DPPH method) of rambai leaves (Baccaurea motleyana Mull. Arg.). Atomic Journal, 4(2), 96–99.
Syam, N. R., Lestari, U., & Muhaimin, M. (2021). Formulation and physical properties test of peel-off gel mask from pure palm oil with carbomer 940 base. Indonesian Journal of Pharma Science, 3(1), 42–55.
Tranggono, R. I. (2007). BP: Cosmetic Science. Gramedia Pustaka Utama.
Zebua, R. D., Syawal, H., & Lukistyowati, I. (2019). Utilization of cherry leaf extract (Muntingia calabura L.) to inhibit the growth of Edwardsiella tarda bacteria. Ruaya Journal, 7(2), 11–20.
